This paper presents results from speech production experiments on English, Korean, and Hindi that compare variation in the acoustic expression of dissimilar phonological laryngeal contrast in stops conditioned by prosodic prominence. Target stops are analyzed from utterance-initial, -medial, and -final positions, with a variation in contrastive focal accent, from the speech data by six male American English speakers, five male Seoul Korean speakers, and five male Delhi Hindi speakers. The results show that prosodic prominence conditions enhanced distinctiveness between contrastive segments in the three languages. The manner in which prosodic prominence and prosodic phrase structure is marked at the level of segmental variation is, however, found to be language-specific to some extent. In addition, a correlation between the size of the phonological inventory and the corresponding acoustic variation was found but the linear correlation was not strongly supported with the findings in the present study.