The spray characteristics of the high speed rotating fuel injection system were studied. The five variants of rotating fuel nozzle were used by spray test. The diameter of single column injection orifices are varied from 1 mm, 2 mm, 3 mm, 4 mm and 5 mm. We constructed high speed rotating test rig and measured droplet size by PDPA (Phase Doppler Particle Analyzer) system. Also spray was visualized by using high speed camera. In the test results, we could understand that length of liquid column from the injection orifice is mainly controlled by the rotational speeds. SMD is decreased with increasing injection orifice diameter and rotational speeds. Furthermore, from the comparison to the theoritical calculation, we confirmed that SMD is influenced by the liquid film thickness which is formed inner surface of injection orifice.