The numerical analysis and safety assessment by construction stages were considered the essential examination particular in order to solving the unstability of long-span bridges in the middle a construction. When estimating structural response characteristics by the construction stage analysis of long-span bridges, the influence of the near-field ground motion (NFGM) would be evaluated as a critical factor for the seismic design because it indicates clearly different aspects from the existing input earthquake motion data. Therefore, this study re-examined the response aspect of long-span bridges considering NFGM characteristics based on the response spectrum result, and advanced the presented numerical analysis program by the related research for conducting the construction stage analysis and reliability assessment of long-span bridges efficiently. The excellency of various construction schemes was assessed using the time history analysis result of critical member considering NFGM characteristics. For evaluating quantitative safety level, the reliability analysis was conducted considering the influence of external uncertainties included in random variables, and presented the safety index and failure probability of the critical construction stage by NFGM characteristics. In addition, the reliability result was examined the influence of internal uncertainties using monte carlo simulation (MCS), and assessed the distribution aspect of the essential analysis result. It is expected that this study will provide the basic information for the construction safety improvement when performing seismic design of long-span bridges considering NFGM characteristics.