The Reference Evapotranspiration Calculator Software (REF-ET) supports computational guidelines for the reference evapotranspiration using seventeen FAO Penman-Monteith (PM) equations simultaneously such as the ASCE and FAO standardized forms. The REF-ET can conveniently consider missing data predictions and regional site characterizations, when reference ET is computed on monthly, daily, and hourly time steps. The applicability of the REF-ET was estimated to simulate the reference ET using hourly weather data from Seoul weather station for 29 years. The result found that the FAO24-Rd and 1957-Makk equations closely concerned with solar radiation parameter which were the most highly correlated to reference ET computed by pan coefficient. In addition, the 1957-Makk equation was identified as the most correct computational method for reference ET by analysis of bias and root mean square error. The 1957-Makk equation could predict the reference ET within the error of less than 1.06 mm/day, though all the other equations tended toward overestimation of predicting the reference ET in comparison with refecence ET of pan. The results of this study suggest that the REF-ET will be applicable to support reference ET estimation for a variety of field condition and time-scale.