The methods developed to measure visual fatigue so far are quite few and lack of validity, and more importantly, they do not figure out the complex properties of the visual fatigue. The purpose of the research was to analyze the factors comprising the visual fatigue and to develop the method to measure it validly. The results are summarized as follows. First, we found that the 3D visual fatigue was comprised of four independent factors (visual stress, eye pain, body pain and image blurring factors). Second, we developed 29 items that measure four factors of 3D visual fatigue. Finally, the watching duration and binocular disparities affected the visual fatigue as had been expected. These results imply that the developed method does measure the three dimensional fatigue validly.