In this study, the most stable portion was identified using 5% moving window during /a/ sustained phonation in normal and pathologic voice signals and the perturbation values were compared between normal and pathologic voices at the mid-point and at the most stable portion using moving window, respectively. The results revealed that some severe pathologic voice signals can be eligible for perturbation analysis by identifying the most stable portion with Err less than 10. In addition, the perturbation acoustic parameters did not differentiate the pathologic voice signals from the normal voice signals when the mid-point was selected to measure the perturbation analysis(p>0.05). However, significantly higher %shimmer and lower SNR values were observed in pathologic voices (p<0.05) when the most stable portion was selected by moving window. In conclusion, moving window could identify the most stable portion objectively which can allow toget the minimum perturbation values (%jitter, %shimmer) and maximum SNR values. Thus, moving window technique can be applicable for more reliable and accurate perturbation acoustic analysis.