In this study, a novel hybrid micro/nano PIV system combining defocusing and TIRFM technique has been developed for the multiscale flow measurement. With the developed system, both far and near field velocity fields have been measured simultaneously in a 2D straight microchannel and the particle trajectories were extracted by the nearest tracking algorithm. The shear rate values taken from experimental results have been estimated by comparing with the analytical solution of 2D Poiseuille flow and it is confirmed that the result shows good agreement with the theoretical value.