The rust fungi are biotrophic fungal pathogens that cause serious disease on Perilla leaves in Korea. The effect of some commonly used chemical preservatives (sorbic acid, D-sorbitol, propionic acid and benzoic acid) against Perilla rust was investigated in this study. Results of this investigation showed that all the preservatives were effective against Perilla rust except benzoic acid. There was no growth of rust spores on the Perilla leaves treated with 0.1% of preservative even after 21 days of preservation. However, 0.01% of preservative also showed remarkable reduction of rust spores on the Perilla leaves as compared to the control groups. Thus, the results of this study indicated that the chemical preservatives used might be useful to control the growth of rust fungi on Perilla leaves.