Effects of temperature ($13{pm}1.5^{circ}C$, $23{pm}1.5^{circ}C$) on the pharmacokinetic properties of oxolinic acid (OA) were studied after oral administration to cultured black rockfish, Sebastes schlegeli. Serum concentrations of OA were determined using HPLC-UV detector after a single dosage of 60 mg/kg body weight (average about 500 g). The peak serum concentrations of OA at $23{pm}1.5^{circ}C$ and $13{pm}1.5^{circ}C$ were $0.60{mu}/ml$ at 30 h and $2.22{mu}g/ml$ at 10 h post-dose, respectively. Better absorption of OA was noted at $13{pm}1.5^{circ}C$ compared to $23{pm}1.5^{circ}C$. The elimination of OA from serum was considerably faster at $23{pm}1.5^{circ}C$ than at $13{pm}1.5^{circ}C$. Both absorption and elimination of OA were affected significantly by temperature. The kinetic profile of absorption, distribution and elimination of OA in serum was analyzed by fitting to a two compartment model, with WinNonlin program. The AUC, Tmax and Cmax at $23{pm}1.5^{circ}C$ were $42.16{mu}g{cdot}h/mell$, 26.13 h and $0.43{mu}g/ml$, respectively. The AUC, Tmax and Cmax at $13{pm}1.5^{circ}C$ were $131.98{mu}g{cdot}h/ml$, 8.81 h and $2.04{mu}g/ml$, respectively.