This study shows that the vertical migration speed of sound scattering layers (SSLs), which is distributed in near Funka Bay, were measured by 3D velocity components acquired from a bottom moorng ADCP. While the bottom mooring type has a problem to measure the velocity vectors of sound scattering layer distributed near to surface, both the continuous vertical migration patterns and variability of backscatterers were routinely investigated as well. In addition, the velocity vectors were compared with the vertical migration velocity estimated from echograms of Mean Volume Backscattering Strength, and estimated to produce observational bias due to SSLs which is composed of backscatterers such as euphausiids, nekton, and fishes have swimming ability.