This paper proposes an image retrieval method based on the Multiple Region-of-Interest. In the proposed method, the image is segmented into blocks, among which the blocks overlapped with multiple ROIs are selected. The similarity of images is measured using the MPEG-7 dominant color descriptor(DCD) and considering the relative location of the overlapped blocks. The experimental results showed that the proposed method improves the retrieval performance than the previous methods using the global DCD or comparing the blocks at the same position. In addition, the method that considers the relative position of blocks overlapped with the multiple ROIs also showed a better performance than the existing methods.