With deer farming now becoming recognized as one of the alternative livestock farming practices, there has been much attention on infectious diseases of cattle, but less awareness of these diseases in deer. Serum samples were randomly collected from 78 deer from 31 farms and 7 wild water deer from wild animal medical center during 2005 to 2007, respectively. A total 85 sera were tested for Neospora caninum antibodies by ELISA. Fourteen farmed deer(17.9%) were positive to N. caninum but no antibodies were found in sera from wild water deer. The sera from all of the area for these study showed positive in the range from 6.7 to 30.7%. On the basis of these study, farmed deer in Jeonbuk province were exposed extensively and seriously to N. caninum which might present a risk to other species included cattle and dog.