Purpose: This study examined the activation of the rectus abdominis (RA), external abdominal oblique (EO), transversus abdominis, internal abdominal oblique (TrA/IO), and Multifidus (MF) muscles while stabilization exercise was performed in a four-point kneeling position. Methods: Experiments were conducted on 21 healthy male adults (mean age=25.29 years) who voluntarily agreed to participate in the study. Each subject was instructed regarding maximal voluntary contractions (MVC) and stabilization exercise in four-point kneeling. While MVC and stabilization exercise of individual muscles were being performed, activation of the muscles was measured using surface electromyography (EMG). Activation of the muscles while performing stabilization exercise in four-point kneeling was normalized to a percentages of the MVC. Results: Left RA, right TrA/IO, right and left MF muscles showed significant differences among the positions. Conclusion: Selecting an optimal position can aid subjects on physical conditions while performing stabilization exercises in the four-point kneeling position.