Purpose: The purpose of this study was to identify the effect of active foot arch support on the muscles of lower extremity electromyographic activity during squat exercise in persons with pronated foot. Methods: The study subjects were 16 persons with pronated foot. They have no history of surgery in lower extremity and trunk and limitation of range of motion or pain when performing squat exercise. Each subject was measured the navicular drop (ND) to determine the pronated foot. And then the subjects were asked to perform three repetitions of a $90^{circ}$ knee flexion squat in both conditions which are 1) preferred squat and 2) squat with active foot arch support. Results: Paired t-test revealed that squat with active foot arch support produced significantly greater EMG activities in abductor hallucis (p=0.00), proneus longus (p=0.03) and gluteus medius (p=0.04) than preferred squat. But the EMG activities of tibialis anterior, vastus medialis oblique and vastus lateralis were not showed significantly different between the both squat conditions. Conclusion: The findings of this study suggest that active foot arch support during squat increase the activities of lower extremity muscles which are the abductor hallucis, proneus longus and gluteus medius. Also, the abductor hallucis which is one of the planter intrinsic muscle and peroneus longus play a role in support of the foot arch and active foot arch support induced the increase of the activity of gluteus medius. Therefore active foot arch support can change the lower extremity biomechanics as well as passive foot support such as foot orthotics and taping.