Development and Application of a Rapid and Simple Loop-mediated Isothermal Amplification Method for Food-borne Salmonella Detection

Development and Application of a Rapid and Simple Loop-mediated Isothermal Amplification Method for Food-borne Salmonella Detection
Development and Application of a Rapid and Simple Loop-mediated Isothermal Amplification Method for Food-borne Salmonella Detection

ㆍ 저자명: Zhao. Xihong,Wang. Li,Chu. Jin,Li. Yanmei,Li. Yanyan,Xu. Zhenbo,Li. Lin,Shirtliff. Mark E.,He. Xiaowei,Liu. Yao,Wang. Jihua,Yang
ㆍ 간행물명: Food science and biotechnology
ㆍ 권/호정보: 2010년|19권 6호|pp.1655-1659 (5 pages)
ㆍ 발행정보: 한국식품과학회
ㆍ 파일정보: 정기간행물|ENG|
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ㆍ 주제분야: 기타

이 논문은 한국과학기술정보연구원과 논문 연계를 통해 무료로 제공되는 원문입니다.

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기타언어초록

A loop-mediated isothermal amplification (LAMP) method for rapid detection of the food-borne Salmonella strains had been developed and evaluated in this study. The optimal reaction condition was found to be $65^{circ}C$ for 45 min, with the detection limit as 1 pg DNA/tube and 100 CFU/reaction. Application of LAMP assays was performed on 214 food-borne Salmonella strains using a rapid procedure and easy result confirmation, where the specificity of LAMP and polymerase chain reaction (PCR) assays was 97.7% (209/214) and 91.6% (196/214), respectively; with a 100% specificity for both assays.

키워드

loop-mediated isothermal amplification (LAMP)Salmonella rapid detection food-borne pathogen invA

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