In this study, antioxidant and anti-photoaging activities of 2-methoxy-5-(2-methyl propyl) pyrazine (MMPP) isolated from peach (Prunus persica (L.) Batsch) were investigated in terms of matrix metalloproteinase (MMP)-1 and type-1 procollagen expression in ultraviolet (UV)-irradiated human skin fibroblasts. MMPP was obtained from the butanol fraction of a 95% ethanol extract of peach. The antioxidant and anti-photoaging activity of MMPP were assessed by 1,1-diphenyl-2-picrylhydrazy (DPPH), Western blot, and reverse trans-cription-polymerase chain reaction (RT-PCR) analyses. MMPP displayed radical scavenging activity, suppressed MMP-1 expression, and increased type-1 procollagen expression. These results support the use of MMPP from peach as an antiphotoaging compound.