Total eight different winter mushroom (Flammulina velutipes) strains were characterized by comparisons of fruiting body morphology, hardness and color after growing at different temperature. Although variations depending on the strains were observed, in general, both stipe length and pilus diameter were shorter while pilus thickness was bigger as growing temperatures were decreased. No significant differences in stipe thickness were observed. The ASI4065 and 4151 had the tallest stipe and the ASI4021 had the shortest stipe. The ASI4103 strain had the biggest pilus diameter. The ASI4153 had most thick pilus, while the ASI 4021 had least thick pilus. Also, the ASI4166 had the most thick stipe and the ASI4065 had the most thin stipe. The hardness of white mushrooms were increased by decreasing growth temperatures, while no relationships between the hardness of brown mushrooms. In most strains, stipe were much stronger than pilei and white mushrooms were harder than brown ones. The strain having most strong stipe was ASI4021. Both ASI 4021 and ASI4166 had the most strong pilus. The color of pilus has much lighter than that of stipe. White mushrooms had high brightness value ranging from 70-90, while brown mushrooms showed low brightness value from 30-70. Saturation value was higher in brown mushrooms. Based on our study, it was shown that environmental conditions had some effects on winter mushrooms, but genetic differences among strains were more important factors to determine most characteristics of winter mushrooms.