현탁액시험법을 사용하여 일반적으로 사용되는 살균소독제 3종(차아염소산나트륨 100ppm 또는 200ppm, 염화알킬($C_{12}-C_{18}$)벤질디메틸암모늄 100ppm 또는 200ppm 및 과산화초산 50ppm 또는 100ppm)의 Escherichia coli ATCC 10536 및 Staphylococcus aureus ATCC 6538에 대한 살균 소독력을 다양한 온도(4~$40^{circ}C$)와 시간(1~60분)에서 청정조건 및 오염조건별로 각각 연구하였다. 차아염소산나트륨(200ppm)은 청정조건에서 $4^{circ}C$, 1분 처리로 E. coli 및 S. aureus를 5 log 이상 감소시켜 3가지 살균소독제 중 가장 효과적인 살균소독제로 나타났다. 그러나 차아염소산나트륨은 간섭물질과의 반응으로 인하여 오염조건에서는 살균소독력이 급속히 감소하였다. 염화알킬($C_{12}-C_{18}$)벤질디메틸암모늄(200ppm)은 사용온도를 높이거나 사용시간을 늘일 경우 살균소독력이 증가하였으며, 저온에서는 살균효과가 낮을 수 있으나 사용시간을 증가시킴으로서 살균소독력을 확보할 수 있었다. 과산화초산(100ppm)은 $4^{circ}C$, 5분 처리로 E. coli 및 S. aureus이 5 log 이상 감소하였다. 살균소독력은 간섭물질에 의해 크게 변하지 않았으며 온도 및 시간변화에 따른 영향도 크지 않았다.
The bactericidal efficacy of three common sanitizers (100 or 200 ppm of sodium hypochlorite, 100 or 200 ppm of n-alkyl($C_{12}-C_{18}$)benzyldimethyl ehloride, and 50 or 100 ppm of peroxyacetie acid) against Escherichia coli ATCC 10536 and Staphylococcus aureus ATCC 6538 was studied using the suspension test method at various exposure temperatures (4~$40^{circ}C$) and times(1~60min) under both dirty and clean conditions, respectively. During the suspension tests, sodium hypochlorite (200 ppm) showed higher bactericidal activity than the other sanitizers under clean conditions, with 5 log reductions against E. coli as well as S. aureus in the first 1 min of treatments at $4^{circ}C$, However, the efficacy of sodium hypochlorite decreased markedly under dirty conditions due to its susceptibility to interfering substances. The efficacy of the n-alkyl($C_{12}-C_{18}$)benzyldimethyl chloride increased considerable as the exposure temperature and time increased. The bactericidal efficacy of the n-alkyl($C_{12}-C_{18}$)benzyldimethyl chloride might be less effective on low temperature, however, the longer time the sanitizer is in contact, the more effective the sanitization effect. Treatment with peroxyacetic acid (100 ppm) showed at least 5 log reduction against E. coli and S. aureus for 5 min at $4^{circ}C$ under both clean and dirty conditions. The efficacy of the peroxyacetic acid was not much altered by interfering substances and aflected by changes in temperature or time.
