This research deals with multiple Gantry-type assembly machines for the optimization of PCB assembly line. The automated assembly machine has 6 nozzles which can linearly move the X axis and the Y axis different from the turret type assembly machine. Each machine is optimized while considering the whole line balancing of three machines in assembly process simultaneously. Simulation models are developed using AutoMod for comparison study with single machine operation cases under various conditions such as types and total number of components to evaluate the proposed method.