In this study the experimental results of fatigue specimen for the Strand Stay Cable Assembly of Extadosed bridges is investigated. The fatigue test and tensile experiment is conducted to 6 kinds of specimens. Test specimen OVM250-31 Strand Cable System manufactured by china OVM B-Machinery Co., Ltd, and OVM250-42 Parallel Strand Stay Cable Assembly manufactured by china OVM B-Machinery Co., Ltd, are passed for fatigue test and rupture tensile test. But Test specimen OVM250-42 Parallel Strand Stay Cable Assembly manufactured by korean A-Machinery Co., Ltd, is not passed for fatigue test conducted according to the "Recommendation for Stay Cable Design. The test result are compared to the fatigue criteria of PHI 2001 for cyclic load, and it is concluded that the current korean design code will be needed for representing the fatigue load in Hot Dip Galvanized Strand Stay Cable. It is verified that the new korean specification and quality criteria of Strand Stay Cable and exact experimental applied process will be needed.