It is really important that trained professional care givers service pursuer for long tenn care insurance in Korea. This study was done to evaluate the infection prevention knowledge and attitude on nursing caregiver students with one group pre test-post test to compare on the education program. The data was collected by standardized self-administered questionnaire with a total of 128 nursing caregiver students in G city. The data was analyzed using the SPSS WIN 10.0 program such as t-test. The knowledge of infection prevention was significantly increased on pre-education and post-education of Infection Prevention Education Program. It is necessary for continuous education to maintain quality of nursing and improve the knowledge of infection prevention and program development on nursing caregiver.