Changes in temperature, loads and boundary conditions may have effects on the dynamic properties of large civil structures. Taking the Run Yang Suspension Bridge as an example, modal properties obtained from ambient vibration tests and from the structural health monitoring system of the bridge are used to identify and evaluate the modal parameter variability. Comparisons of these modal parameters reveal that several low-order modes experience a significant change in frequency from the completion of the bridge to its operation. However, the correlation analysis between measured modal parameters and the temperature shows that temperature has a slight influence on the low-order modal frequencies. Therefore, this paper focuses on the effects of the boundary conditions on the dynamic behaviors of the suspension bridge. An analytical model is proposed to perform a sensitivity analysis on modal parameters of the bridge concerning the stiffness of expansion joints located at two ends of bridge girders. It is concluded that the boundary conditions have a significant influence on the low-order modal parameters of the suspension bridge. In addition, the influence of vehicle load on modal parameters is also investigated based on the proposed model.