In this study, we analyzed the pulse-duration, pulse-interval and peak-frequency of echolocation call in three species as Rhinolophus ferrumequinum, Pipistrellus abramus, and Myotis macrodactylus. The peak frequency and pulse duration for above mentioned species were 69 kHz, 47 kHz and 49 kHz and $69.39{pm}8.76;ms$, $4.95{pm}0.77;ms$ and $3.09{pm}0.48;ms$ for R. ferrumequinum, P. abramus and M. macrodactylus, respectively. The pulse intervals for R. ferrumequinum, P. abramus and M. macrodactylus were $103.61{pm}9.05;ms$, $67.59{pm}3.47;ms$ and $66.35{pm}4.96;ms$, respectively. The pulse pattern of R. ferrumequinum was setting into a short FM call and linked to long CF call and went through the short FM call again. The pulse pattern of M. macrodactylus was comprised with serial short FM call and the CF call was not checked up in accordance with the spectrogram analysis. The long FM call and short CF call got join together for the P. abramus and the peak frequency was checked up at the pulse ending as CF call.