In this paper, we study on an optimal design of a triple-band PIFA (Planar Inverted-F Antenna) of 433 MHz, 912 MHz and 2.45 GHz by using evolution strategy. Generally, the resonant frequency of the PIFA is determined by the width and length of a U-type slot used. However the resonant frequencies of the multiple U slots are varied by the mutual effect of the slots. Thus the optimal width and length of U-type slots are determined by using an optimal design program based on the evolution strategy. To achieve this, an interface program between a commercial EM analysis tool and the optimal design program is constructed for implementing the evolution strategy technique that seeks a global optimum of the objective function through the iterative design process consisting of variation and reproduction. The resonant frequencies of initial model are 439.5 MHz, 981.5 MHz and 2.563 GHz. However, the resonant frequencies of the triple-band PIFA yielded by the optimal design program are 430.5 MHz, 907 MHz and 2.4515 GHz. Measured resonant frequencies are 433.5 MHz, 905.5 MHz and 2.454GHz, which show a good agreement with the simulation results.