Purpose: Hyperglycemia is associated with an risk of cardiovascular disease, mortality, diabetes mellitus and musculoskeletal disorders. The purpose of this study was to analyse the effect of two different treadmill exercise on blood levels of glucose in SD rats. Methods: The experimental groups were divided into 2 groups. The exercise was performed in the treadmill for 20minutes with 15m/min(group I, n=20) and 25m/min(group II, n=20) Blood samples were collected before exercise, 6day and 12days after treadmill exercise. Results: A significant difference was only at 12days on independent samples t-test for both groups. There were statistically significant difference between pre and 6days, 6days and 12days, pre and 12days on tests of pairwise comparisons for each groups. Both group were showed linear effects following treadmill exercise in 12days. Conclusion: These results suggest that a trend toward decrease in the levels of glucose, following treadmill exercise, were revealed in both group. Aerobic exercise related to a positive effect in control of glucose level.