This study was performed to development a dental three-dimensional laser scanning system and measure the accuracy of new adjustable upper dental impression tray. The metal stock, individual, and new adjustable stock trays were used for 30 stone casts(10 casts each) duplicated a resin master model of maxilla. The dental stone was poured in a vinyl polysiloxane impressions and allowed to set for on hour. The master model and the duplicated casts were digitized using an dental scanning system. The distance between the reference points were measured and analyzed on the graphic image of 3D graphic software of CATIA. The statistical significance of the differences between the groups was determined by a two-way ANOVA. There were no significant differences between the accuracies of the adjustable stock tray and the master model except only anterior arch width on the upper arch. The adjustable upper stock tray showed clinically acceptable accuracies of the study cast produced by them.