Roses are among the most economically important ornamental crops. Inter-simple sequence repeat (ISSR) markers were used to study phylogenetic relationships among 7 rose species. Among 9 ISSR primers, 6 primers showed acceptable polymorphism and amplified 66 bands of which 50 were polymorphic. Banding patterns were transformed into binary data of presence-absence and matrices were processed using NTSYS pc 2.02 software program. The dendrogram was constructed using Jaccard coefficient and UPGMA algorithm. The 7 rose species were classified into 3 major groups with within-group similarity values of >0.58. Group 1 included $R.$ $banksiae$ group 2, $R.$ $canina$, $R.$ $chinensis$, $R.$ $damascena$, $R.$ $moschata$ and $R.$ $hybrida$ and group 3, $R.$ $foetida$. None of the species used in this study clustered within group 1, indicating that there is no direct relationship between these species. The species that were placed with $R.$ $hybrida$ in group 2 are the ancestors of $R.$ $hybrida$ and because of other species except these four species have contributed to the gene pool of modern garden roses, 59% similarity between these roses in this study was expected. In this phylogenetic tree $R.$ $foetida$ clusters in the lower part of the tree showed 49% similarity with group 2.