In present study, classification and quality control of Genus Polygonatum were developed using the isolated from Polygonati Odorati Rhizoma and Polygonati Rhizoma. 3 components were isolated from Butanol fractions of Polygonati Rhizoma, and 2 components were isolated from Hexane and Butanol fractions of Polygonati Odorati Rhizoma. All the components were obtained using silica gel and ODS column chromatography. The compounds were identified as adenosine, 14-hydroxylfurost-5-ene-3-O-${eta}$-D-glucopyranosyl-($1{ ightarrow}2$)-O-${eta}$-D-glucopyranosyl-($1{ ightarrow}4$)-O-${eta}$-D-galactopyranosyl-26-O-${eta}$-D-glucopyranoside, 22-O-methyl-14-hydrocxyfurost-5-ene-3-O-${eta}$-D-glucopyranosyl-($1{ ightarrow}2$)-O-${eta}$-D-glucopyranosyl-($1{ ightarrow}4$)-O-${eta}$-Dgalactopyranosyl-26-O-${eta}$-D-glucopyranoside, ${eta}$-Sitosteryl-3-O-${eta}$-D-D-glucopyranoside, 14-hydoxylfurost-5-ene-3-O-${eta}$-Dglucopyranosyl-($1{ ightarrow}2$)-O-[${eta}$-D-xylopyranosyl-($1{ ightarrow}3$)]-O-${eta}$-D-glucopyranosyl-($1{ ightarrow}4$)-O-${eta}$-D-galactopyranoside through physicochemical data, spectroscopic methods ($^1H$-NMR, $^{13}C$-NMR, Mass) according references. The quality control of genus Polygonatum were conducted using HPLC quantitative analysis of 14-hydroxylfurost-5-ene-3-O-${eta}$-D-glucopyranosyl-($1{ ightarrow}2$)-O-${eta}$-D-glucopyranosyl-($1{eta}4$)-O-${eta}$-D-galactopyranosyl-26-O-${eta}$-D-glucopyranoside, 14-hydoxylfurost-5-ene-3-O-${eta}$-D-glucopyranosyl-($1{ ightarrow}2$)-O-[${eta}$-D-xylopyranosyl-($1{ ightarrow}3$)]-O-${eta}$-D-glucopyranosyl-($1{ ightarrow}4$)-O-${eta}$-D-galactopyranoside in 30 samples collected throughout Korea and China. This method provided a tool for standardization of mix or misusing the commercial Odorati Rhizoma and Polygonati Rhizoma. As a result, contained quantity of 14-hydroxylfurost-5-ene-3-O-${eta}$-D-glucopyranosyl-($1{ ightarrow}2$)-O-${eta}$-D-glucopyranosyl-($1{ ightarrow}4$)-O-${eta}$-D-galactopyranosyl-26-O-${eta}$-D-glucopyranoside was measured $0.008{pm}0.006%$ and 14-hydoxylfurost-5-ene-3-O-${eta}$-D-glucopyranosyl-($1{ ightarrow}2$)-O-[${eta}$-D-xylopyranosyl-(13)]-O-${eta}$-D-glucopyranosyl-($1{ ightarrow}4$)-O-${eta}$-Dgalactopyranoside was measured $0.026{pm}0.012%$.