Purpose: This study investigated the effectiveness of AIDS education on knowledge, prejudice, susceptibility, severity, and intention of safe sex behavior among university students. Methods: This study was conducted from March, 2008 to May, 2008. The research design was a non-equivalent control group pre-post test. Participants 78 university students (control group) and 89 university students (experimental group). For the latter, AIDS education was provided once weekly for 1 h for 4 weeks. The educational program included understanding of sexuality, facts of AIDS, life stories of patients with AIDS, and safe sex behavior. The differences between two groups after education were analyzed by independent t-test. Results: The experimental group had significantly higher scores for knowledge, prejudice, susceptibility, and intention of safe sex behavior compared to the control group. However, no significant difference was found for severity between two groups. Conclusion: AIDS education can be effective in improving knowledge, prejudice, susceptibility, and intention of safe sex behavior, and is recommended as an effective AIDS education for university students.