Twenty five compounds including ten triterpenes (1-3, 5-11), six flavonoids (12-15, 24, 25), five lignans (17, 18, 21-23), two butenyl clohexnone glycosides (19-20), one fructofuranoside (16) and one fatty acid (4) were isolated from the roots of Ulmus davidiana var. japonica. The structures of those compounds were identified by comparing their physicochemical and spectral data with those of published in literatures. All the compounds were evaluated for DNA topoisomerase inhibitory activities and cytotoxicities. Among the purified compounds, 4 and 19 showed more potent inhibitory acitivities ($IC_{50}$: 39 and 19 ${mu}M$, respectively) than camptothecin, as the positive control ($IC_{50}$: 46 ${mu}M$) against topoisomerase I. Compounds, 4, 10, 12, 19, 24 and 25 showed strong inhibitory activities toward DNA topoisomerase II ($IC_{50}$: 0.1, 0.52, 0.47, 0.42, 0.17 ${mu}M$ and 17 nM, respectively), which were more potent than that of etoposide as positive control ($IC_{50}$: 20 ${mu}M$). In A549 cell line, 5 and 6 showed cytotoxicities ($IC_{50}$: 4 ${mu}M$ and 3 ${mu}M$, respectively, with $IC_{50}$ of camptothecin as positive control: 10.3 ${mu}M$). In the HepG2 cell line, 3, 5 and 7 showed cytotoxicity ($IC_{50}$: 4, 3 and 4 ${mu}M$, respectively, with $IC_{50}$ of camptothecin: 0.3 ${mu}M$). Compounds 6, 12 and 23 showed cytotoxicities in the HT-29 cell line ($IC_{50}$: 19, 19 and 15 ${mu}M$, respectively, with $IC_{50}$ of camptothecin: 2 ${mu}M$).