The effects of glass beads on the rheological properties of culture broths of Acremonium chrysogenum M35 grown in baffled flasks was investigated in this study. The addition of glass beads to cultures of A. chrysogenum M35 led to considerably higher levels of shear rheological properties such as shear stress versus shear rate, which are essential for controlling the performance of culture broths. The shear properties of culture broths of A. chrysogenum M35 evaluated in this study were also found to be better predicted by the powerlaw and the Herschel-Bulkley fluid models than by the Bingham plastic and the Casson fluid models. The consistency index (K) and the flow behavior index (n) data from the power-law fluid model for various culture broths clearly substantiated the usefulness of glass beads for effectively promoting the cell growth and cell differentiation, because they were directly affected by the morphological changes of A. chrysogenum M35 induced by the glass beads.