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Isolation and Characterization of Lactic Acid Bacteria Strains with Ornithine Producing Capacity from Natural Sea Salt
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  • Isolation and Characterization of Lactic Acid Bacteria Strains with Ornithine Producing Capacity from Natural Sea Salt
  • Isolation and Characterization of Lactic Acid Bacteria Strains with Ornithine Producing Capacity from Natural Sea Salt
저자명
Yu. Jin-Ju,Oh. Suk-Heung
간행물명
The journal of microbiology
권/호정보
2010년|48권 4호|pp.467-472 (6 pages)
발행정보
한국미생물학회
파일정보
정기간행물|ENG|
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이 논문은 한국과학기술정보연구원과 논문 연계를 통해 무료로 제공되는 원문입니다.
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기타언어초록

Two lactic acid bacteria (LAB) having ornithine-producing capacity were isolated from Korean natural sea salt. They were Gram-positive, short rod-type bacteria, and able to grow anaerobically with $CO_2$ production. The isolates grew well on MRS broth at 30-37$^{circ}C$ and a pH of 6.5-8.0. The optimum temperature and pH for growth are $37^{circ}C$ and pH 7.0. The isolates fermented D-ribose, D-galactose, D-lactose, D-maltose, D-cellobiose, D-tagatose, D-trehalose, sucrose, D-melezitose, gentiobiose, D-glucose but not D-melibiose, inositol, and L-sorbose. The 16S rDNA sequences of the two isolates showed 99.5% and 99.6% homology with the Weissella koreensis S5623 16S rDNA (Access no. AY035891). They were accordingly identified and named as Weissella koreensis MS1-3 and Weissella koreensis MS1-14, and produced intracellular ornithine at levels of 72 mg/100 g cell F.W. and 105 mg/100 g cell F.W. and extracellular ornithine at levels of 4.5 mg/100 ml and 4.6 mg/100 ml medium, respectively, by culturing in MRS broth supplemented with 1% arginine. High cell growth was maintained in MRS broth with a NaCl concentration of 0-6%. These results show for the first time that Korean natural sea salts contain lactic acid bacteria Weissella koreensis strains having ornithine producing capacity.