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Isolation of Synthetic Lethal Mutations in the rsm1-null Mutant of Fission Yeast
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  • Isolation of Synthetic Lethal Mutations in the rsm1-null Mutant of Fission Yeast
  • Isolation of Synthetic Lethal Mutations in the rsm1-null Mutant of Fission Yeast
저자명
Moon. Dong-Ge-Ra-Mi,Park. Yun-Sun,Kim. Cha-Yeon,Yoon. Jin-Ho
간행물명
The journal of microbiology
권/호정보
2010년|48권 5호|pp.701-705 (5 pages)
발행정보
한국미생물학회
파일정보
정기간행물|ENG|
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이 논문은 한국과학기술정보연구원과 논문 연계를 통해 무료로 제공되는 원문입니다.
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기타언어초록

To identify mutations in genes that are genetically linked to rsm1, we performed a synthetic lethal genetic screen in the fission yeast, Schizosaccharomyces pombe. Four mutations that showed synthetic lethality in combination with the rsm1null allele were isolated from approximately 320,000 colonies and defined in three complementation groups. One mutant (SLrsm1) exhibited a significant accumulation of poly(A)$^+$ RNA in the nucleus under synthetic lethal conditions, while the rest had no mRNA export defects. In addition, some genes (spmex67, rae1, or mlo3) required for mRNA export complemented the growth defects of the identified mutants. These results suggest that the isolated mutants contain mutations in genes that are involved in mRNA export and/or pre-mRNA retention.