This study investigated the effects of DO concentration on DHA fermentation and of DO-stat fed-batch fermentation using a pH control strategy, on 1,3-dihydroxyacetone (DHA) production. The results showed that DO-stat fed-batch fermentation with pH-shift control was the optimal bioprocess for DHA production. DO-stat fedbatch fermentation was carried out at 30% air saturation, and the culture pH was automatically maintained at pH 6.0 during the first 20 h and then shifted to pH 5.0 until the end of the fermentation. An optimal DHA concentration of $175.9{pm}6.7$ g/L, with a production yield to glycerol of $0.87{pm}0.04$ g/g, was obtained at 72 h of DO-stat fed-batch fermentation at $30^{circ}C$ in a 15 L fermenter.