Durability performance evaluation technology using Virtual Testing Method is a new concept of a vehicle design, which can reduce the automotive components design period and cost. In this paper, the fatigue life of an aluminum knuckle of a passenger car is evaluated using virtual testing method. The flexible multibody dynamic model of a front half car module is generated and solved with service loads which are measured from Belgian roads. Using a multibody dynamic analysis software, the flexible multibody dynamic simulation of a half car model is carried out and the dynamic stress profile of an aluminum knuckle is acquired. The stress profile is exported to a fatigue analysis software and durability performance of an aluminum knuckle is evaluated.