If the vibration is occurred in a large-sized bus roof, it makes people annoying and complaining the quality of a large-sized bus. So in design stage, it must be considered. To assess vibration at the roof which is equipped with air conditioner in design stage, finite element model is constructed. Computer simulation analysis and experimental method are performed. The dynamic characteristics of the large-sized bus are found by using eigenvalue method. It is related with dynamic behavior. The running conditions of a large-sized bus are velocity and road condition which followed experimental conditions. And the frequency response of a large-sized bus is well correlated with analysis result. Modal participation method is used for finding major modes at each peak. Using this method, we found that front and rear suspension system, engine mounting system and roof structure are the major reasons of the roof vibration. To reduce vibration level of roof in a large-sized bus, spring stiffness of front and rear suspension system, spring stiffness of engine mounting system and roof structure are properly combined. From this study, the vibration characteristics of the roof structure of a large-sized bus can be to a satisfactory level.