This study was carried out to find the effect on growing media and proper time for water supply in rice seedling stages. Plant height of rice seedling for SIPAN-BRL among current several growing medias was higher in Chuchungbyeo than Ilpumbyeo, and that of root length was higher in check among used growing medias. Fresh and dry weight of shoot and root of two rice cultivar. Water time to absorption on each treatment of growing media concentration took similarly in SIPAN-BNH and check were similar. But 500 minutes did in 100ppm and did 370 and 470minute in 300 and 500ppm, respectively. Wilting time of rice seedling was appeared highly at 300ppm of SIPAN-BRL in Chuchungbyeo and 100ppm of SIPAN-BRL in Ilpumbyeo, while it prolonged at 300ppm in Chuchungbyeo and 500ppm in Ilpumbyeo, respectively. Water content at wilting stage was appeared highly at SIPAN-BRL 500ppm in both Chuchungbyo and Ilpumbyeo, while SIPAN-BNL was high in 300ppm of Chuchung-byeo and Ilpumbyeo, respectively. Plant and root length of SIPAN-BNL treated with 300ppm in 10day-rice seedling were highly appeared, while dry weight of shoot was high at 300ppm SIPAN-BNL.