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Biodegradation of Endosulfan by Klebsiella oxytoca KE-8 Immobilized on Activated Carbon
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  • Biodegradation of Endosulfan by Klebsiella oxytoca KE-8 Immobilized on Activated Carbon
  • Biodegradation of Endosulfan by Klebsiella oxytoca KE-8 Immobilized on Activated Carbon
저자명
Jo. Min-Sub,Lee. Jung-Bok,Kim. Jang-Eok,Sohn. Ho-Yong,Jeon. Chun-Pyo,Choi. Chung-Sig,Kwon. Gi-Seok
간행물명
한국환경농학회지
권/호정보
2010년|29권 2호|pp.176-183 (8 pages)
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한국환경농학회
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정기간행물|ENG|
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이 논문은 한국과학기술정보연구원과 논문 연계를 통해 무료로 제공되는 원문입니다.
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기타언어초록

Endosulfan degrading ability of Klebsiella oxytoca KE-8 immobilized by entrapment with activated carbon was examined. Endosulfan degradation by the immobilized bacterial strains on several different activated carbon based support materials was investigated. Based on results, activated carbon ($8 imes30$ mesh) was chosen as a support material. The immobilized Klebsiella oxytoca KE-8 with the cell density of 4 mg $g^{-1}$ (dry weight) degraded 22.18 ug $ml^{-1}$ endosulfan within 5 days at pH 7.0, $30^{circ}C$ in batch shake flask cultures. Also, we an experimented recycle packed bed column mode and continuous packed bed column mode for endosulfan degradation. Under optimum operation condition, the immobilized cells in a laboratory scale pack bed column with support beads were able to degrade endosulfan completely in defined minimal salt medium at a maximum rate of 129.6 ug $ml^{-1}$ per day. Moreover, the endosulfan degradation activity could be demonstrated at $4^{circ}C$ for one month without significant decrease in activity. Results of this study suggest that immobilized cells of Klebsiella oxytoca KE-8 might be applicable to endosulfan contaminated site.