The effect of fuel injection characteristics on engine performance has been known for improving fuel economy and emission reduction. In this study, the spray characteristics of crude palm oil blended fuel with conventional diesel fuel was investigated. The experiments were performed to evaluate the effect of crude palm oil blending ratio and injection pressure on the spray behavior. The droplet size of injected fuel was analyzed through laser diffraction particle analyzer (LDPA). Also, spray atomization characteristics were investigated in terms of Sauter mean diameter (SMD) and droplet distribution at various injection conditions. Fuel containing crude palm oil has different spray pattern on account of the high viscosity. Through those experimental results, we found that the increase of blending ratio made droplet size larger, SMD of biodiesel 100% was increased 30.2% than that of diesel fuel 100% under injection pressure of 60 MPa.