Post-Harvest processing engineering is a field that studies prevention of the quality change of agricultural products during sorting, packaging, storage, and distribution after harvested. In distribution steps, agricultural products could be damaged by physical force, it is the main reason of low quality and they lost value of commodities. This study was performed to find the vibration characteristics of the peach, and to find the extent of the damage on the peach by fatigue stress. The vibration data was obtained on expressway and the vibration characteristics of peach was used to find the damage on the peach. To analyze the vibration characteristics of peach, the resonance frequency and vibration transmissibility were measured. The resonance frequency of the peach was 167.98 Hz and the transmissibility was 4.06 at resonance point. It was 150 ~ 250 Hz that the transmissibility was more than 1. And the transmissibility in simulated test was measured. When the trasmissibility was more than 1, the range was 15 ~ 65 Hz, and when it was less than 1, the range was 65 ~ 175 Hz. When the transmissibility was about 1, the range was 5 ~ 15 Hz. The damage and the vibration cycle numbers of peaches were compared with input frequency and acceleration. More damage and less cycle number happened in 30 Hz than in 62.5 Hz. The reason was that the transmissibility of 30 Hz was higher and the vibration displacement in lower frequency was more. The more acceleration and cycle number increased, the more the bruising volume of peaches increased. The bruising volume ratio for vibration fatigue was measured according to input acceleration and cycle number. Using measured data, regression models for bruising volume ratio(BVR) was developed as a function of the acceleration(A) and cycle number(CN) as follows. BVR = a * $A^b*$ $(CN)^c$