치주염이나 구취발생과 관련된 구강미생물에 대한 저출력레이저의 살균효과를 관찰하기 위하여, Porphyromonas gingivalis 2561 (P. gingivalis 2561), Prevotella intermedia (Pr. intermedia)에 광감수성증가물질을 처리함으로써 실험균주의 광민감성을 증가시킨 후 저출력레이저 650nm의 파장을 연속적으로 1분, 2분, 3분, 5분간, 각각 1cm, 2cm, 3cm 거리에서 조사하여 다음과 같은 결과를 얻었다. 1. P. gingivalis 2561의 경우 Toluidine blue O(TBO) 처리 후 650nm의 파장으로 3cm의 거리에서 1분간 조사한 실험군은 TBO만 처리한 실험대조군에 비해 99.99% 이상의 항균효과를 나타내었다. 2. Pr. intermedia의 경우 TBO 처리 후 650 nm의 파장으로 3 cm의 거리에서 1분간 조사한 실험군은 TBO만 처리한 실험대조군에 비해 99.8% 이상의 항균효과를 나타내었다. 3. P. gingivalis 2561의 경우 TBO 처리 후 650 nm의 파장을 연속으로 각각 1분, 2분, 3분간 조사한 실험군의 경우 시간이 길어질수록 살균효과가 점차 증가되었다. 4. P. gingivalis 2561의 경우 조사거리가 1 cm, 2 cm, 3 cm으로 증가할수록 살균효과가 약간 감소되었다. 5. Pr. intermedia의 경우 조사거리가 1 cm, 2 cm, 3 cm으로 증가할수록 살균효과가 약간 감소되었다. 위와 같은 결과를 바탕으로 저출력레이저의 살균효과는 조사시간이 길어질수록, 조사거리가 짧을수록 살균효과가 증가되었는데, 살균효과가 가장 적게 나타난 3 cm의 거리에서 1분간 조사한 실험군에서도 TBO만 처리한 경우보다 99.8% 이상의 살균효과를 나타내었으므로 저출력레이저의 구강미생물에 대한 살균효과는 명백하다고 생각된다. 따라서 photosensitizer의 부작용이 없는 한계 내에서 사용한다면 저출력레이저가 치주염, 임플란트주위염 등에 대한 보조적 치료와 구취조절 등에 매우 유용하게 사용되리라고 생각된다.
This study is to observe the bacteriocidal effect of the Low Level Laser (LLL) against oral microorganisms which are related to the occurrence of periodontal diseases and oral malodors. The Porphyromonas gingivalis 2561 (P. gingivalis 2561) and Prevotella intermedia (Pr. intermedia) were treated with photosensitizing substance-toluidine blue O (TBO; C.I. 52040) and then radiated with the LLL which has 650nm wavelength for 1, 2, 3 and 5mins. continuously upon varying distances of 1, 2 and 3cm for each experimental groups. The results are as follows; 1. The P. gingivalis 2561 which was treated with TBO and then radiated with LLL at a distance of 3cm for 1min. showed 99.99% higher antibacterial effect in comparison to the experimental group treated only with TBO. 2. The Pr. intermedia which was treated with TBO and then radiated with LLL at a distance of 3cm for 1min. showed 99.8% higher antibacterial effect in comparison to the experimental group treated only with TBO. 3. The bacteriocidal effect of the P. gingivalis 2561 treated with TBO was found to gradually increase as the radiation time of LLL extended from 1min. to 3min. at 1min. intervals. 4. A slight decrease in bacteriocidal effect of the P. gingivalis 2561 was found as the radiation distance of LLL increased from 1cm to 3cm at 1cm intervals. 5. The bacteriocidal effect of the Pr. intermedia was found to slightly decrease as the radiation distance of LLL increased from 1cm to 3cm at 1cm. intervals. As the results shown above suggest, the bacteriocidal effect of LLL was found to increase as the radiation time extended and the distance shortened. Moreover, even the experimental group radiated with LLL at 3cm distance for 1min. which showed the lowest level of bacteriocidal effect, was found to have 99.8% higher bacteriocidal effect than the experimental group which was treated only with TBO and, therefore, this clearly shows the bacteriocidal effect of LLL against oral microorganisms. Thus, the use of LLL is thought to become very useful for suportive treatment for periodontitis and implantitis, and controlling oral malodors as long as it is used within the limits where there is no side effect.
