High contrast and high resolution are the most important factors for examining mammography images. Despite of the inconveniences of screen-film, most clinics still prefer them to computed radiography(CR) and direct radiography(DR). The reading of screen-film mammography images is influenced by the brightness from the X-ray illuminator, the exam room and incoming light from outside sources. Therefore, a comparative analysis on the results of mammo phantom images would be variated by the changes in the reading environment. There was no influence on reading results from the examiners close distance eyesight(p > 0.05); however, reading of micro lesions improved with greater darkness in the X-ray film reading room and the brightness of the X-ray illuminator(p < 0.05). Also, observation of fiber and mass images were maximized at a distance of 50 cm from the reader. Now, it is possible to observe these small classification groups using a magnifying glass without being physically close to the image. For the image of mammography, obtaining high quality images is important but in order to get an accurate clinical lesions of the reading also needs to be considered the optimal environmental factors.