The goal of this study is to analysis natural frequency for different static postures of human leg. To perform this research human leg is modeled by multi-body modeling for the musculoskeletal system. This leg model has biarticular muscles which acting on two joints and the muscles represents some of the major muscles, such as hamstring, of the upper and lower limbs. To obtain each static equilibrium position energy method is employed and to analysis natural frequency linearization method for constrained mechanical system is employed. Static equilibrium position depends on some parameter or condition such as hamstring stiffness or external force. Making a change these parameter the aim of this research can be performed.