Objectives : The purpose of this study is to investigate the effect of Bacillus-fermented Scutellariae Radix acupuncture solution (SB) on interleukin(IL) production in mouse macrophage stimulatedby lipopolysaccaride(LPS). Methods : Productions of interleukins were measured y High-throughput Multiplex Bead based Assay with Bio-plex Suspension Array System based on $xMAP^{(R)}$(multi-analyte profiling beads) technology. To begin with, cell culture supernatant was obtained after treatment with LPS(1 ${mu}g/mL$) and SB for 24 hour. Then, it was incubated with the antibody-conj${mu}g$ated beads for 30 minutes. And detection antibody was added and incubated for 30 minutes. After incubating for 30 minutes, Strepavidin-conjugated Phycoerythrin(SAPE) was then added. Incubating for another 30 minutes, the level of SAPE fluorescence was analyzed on Bio-plex Suspension Array System. Results : The results of the experiment are as follows. SB significantly inhibited the LPS-induced production of IL-3($9.15{pm}0.35$ pg/mL) by $6.92{pm}0.05,;7.21{pm}0.11,;6.96{pm}0.33,;and;7.45{pm}0.74$ pg/mL at the concentration of 25, 50, 100, and 200 ${mu}g/mL$ in mouse macrophage RAW 264.7 cells (p<0.05). SB significantly inhibited the LPS-induced production of IL-5($7.30{pm}0.48$ pg/mL) by $6.50{pm}0.29,;6.30{pm}0.25,;6.30{pm}0.25,;and;5.80{pm}0.25$ pg/mL at the concentration of 25, 50 100, and 200 ${mg}g/mL$ in RAW 264.7 cells (p<0.05). SB significantly inhibited the LPS-induced productiion of IL-9($17.26{pm}0.19$ pg/mL) by $15.01{pm}0.43$ pg/mL at the concentration of 25 ${mu}g/mL$ in RAW 264.7 cells(p<0.05). SB significantly inhibited the LPS-induced productioh of IL-13($187.80{pm}2.90$ pg/mL) by $152.80{pm}4.25,;172.80{pm}3.97,;162.10{pm}6.67,;and;165.30{pm}11.80$ pg/mL at the concentration fo 25, 50, 100, and 200 ${mu}g/mL$ in RAW 264.7 cells(p<0.05). SB significantly inhibited the LPS-induced production of IL-17($18.30{pm}0.95$ pg/mL) by $13.30{pm}1.25,;13.80{pm}1.11,;13.30{pm}0.75,;and;14.00{pm}1.08$ pg/mL at the concentration of 25, 50 100, and 200 ${mu}g/mL$ in RAW 264.7 cells(p<0.05). SB significantly inhibited the LPS-induced production of IL-23($43.90{pm}0.83$ pg/mL by $39.50{pm}1.26,;38.00{pm}1.78,;and;39.60{pm}2.49$ pg/mL at the concentration of 25, 100, and 200 ${mu}g/mL$ in RAW 264.7 cells(p<0.05). Conclusions : These results suggest that SB has anti-inflammatory activity related with its inhibition of IL-3, IL-5, IL-13, IL-17, and IL-23 production in macrophages.