We verified that the apoptosis activities were examined by DNA fragmentation, flow cytometric analysis with annexin V staining, activation of caspase-3, and cytochrome c release. In the result, $alpha$- and $eta$-eudesmol induced DNA fragmentation in HL-60 cells at a concentration of $80;{mu}M$, respectively. Additionally, pro-apoptotic cells sorted by flow cytometry analysis were detected in HL-60 cells to 31.77 and 29.67% with $acute{a}$- and $eta$-eudesmol of $80;{mu}M$. Thus, both $alpha$- and $eta$-eudesmol exerted caspase-3 activation and cytochrome c release at $80;{mu}M$ in HL-60 cells. These results are firstly reported that the sesquiterpenes, $alpha$- and $eta$-eudesmol are apoptosis inducers through mitochondria-dependent caspase cascade in HL-60 cells.