A mobile direct detection Doppler LIDAR based on molecular backscattering for measurement of wind in the stratosphere has been developed in Hefei, China. First, the principle of wind measurement with direct detection Doppler LIDAR is presented. Then the configuration of the LIDAR system is described. Finally, the primary experimental results are provided and analyzed. The results indicate that the detection range of the designed Doppler LIDAR reached 50 km altitude, and there is good consistency between the molecular Doppler wind LIDAR(DWL) and the wind profile radar(WPR) in the low troposphere.