KC-100 is a 4 seats, single piston engine, civil aircraft whose type certificate is applied for KAS 23 (FAR 23) for the first time in Korea. Its system safety assessment and analysis have been conducted to meet the minimum safety requirement in KAS 23 and to verify the safety of equipment, system, and installation in accordance with the requirement of ${S}$23.1309 and the guidelines in FAA AC 23.1309-1D and SAE ARP 4761. This safety assessment begins with the FHA (Functional Hazard Assessment) at aircraft and system level in preliminary design phase, and all of the safety assessment and analysis reports including the preliminary version of SSA (System Safety Assessment) have been prepared during detail design phase. The revised version of these safety reports will be approved by Airworthiness Authority through the ground and flight test phases. In this paper, the safety assessment requirement in ${S}$23.1309, safety assessment guideline in AC 23.1309-1D, and safety assessment and analysis methods in ARP 4761 will be explained based on the application example for KC-100 development. The experience and knowledge of this system safety assessment for civil aircraft can be applied to commuter aircraft of FAR 23 class or large transport airplane of FAR 25 class.