Purpose : The purpose of this study was to investigate the VMO/VL ratio and onset timing using EMG biofeedback training over a 5-day period. Methods : Twenty-one healthy female college students with no known right knee musculoskeletal dysfunction were recruited this study. Muscle activity was measured by surface electromyography(Myosystem 1400A, Noraxon Inc., U.S.A). Statistical analysis was used two-way repeated ANOVA to know difference between the vastus lateralis and vastus medialis oblique onset timing differences, VMO/VL ratio. Results : Biofeedback training group significantly improved VMO/VL ratio and EMG activity of the vastus medialis oblique after intervention. Conclusion : These result indicate that biofeedback training on the vastus medialis oblique has effect on the VMO/VL ratio. EMG biofeedback can be recommended for the facilitation of VMO muscular recruitment.