In this paper, a dynamic GTS allocation method of wireless control networks is proposed for the use of factory automation using IEEE 802.15.4 MAC protocol. A superframe of IEEE 802.15.4 is applied to the transmission method of real-time periodic I/O data of wireless control systems within the limited time in factory environment. The method is proposed for efficient transmission of real-time periodic I/O traffic. The simulation results show the average network utilization and available I/O node numbers could be increased by the proposed method.