기관회원 [로그인]
소속기관에서 받은 아이디, 비밀번호를 입력해 주세요.
개인회원 [로그인]

비회원 구매시 입력하신 핸드폰번호를 입력해 주세요.
본인 인증 후 구매내역을 확인하실 수 있습니다.

회원가입
서지반출
Archaeal Diversity During Composting of Pig Manure and Mushroom Cultural Waste Based on 16S rRNA Sequence
[STEP1]서지반출 형식 선택
파일형식
@
서지도구
SNS
기타
[STEP2]서지반출 정보 선택
  • 제목
  • URL
돌아가기
확인
취소
  • Archaeal Diversity During Composting of Pig Manure and Mushroom Cultural Waste Based on 16S rRNA Sequence
  • Archaeal Diversity During Composting of Pig Manure and Mushroom Cultural Waste Based on 16S rRNA Sequence
저자명
Lee. Young-Han,Kim. Sung-Kyum,Kim. Yong-Ho,Jeong. Yu-Seok,Yun. Myoung-Geun,Cho. Ji-Joong,Kim. Jong-Min,Yun. Han-Dae,Kim. Hoon
간행물명
Journal of the Korean Society for Applied Biological Chemistry
권/호정보
2010년|53권 2호|pp.230-236 (7 pages)
발행정보
한국응용생명화학회
파일정보
정기간행물|ENG|
PDF텍스트
주제분야
기타
이 논문은 한국과학기술정보연구원과 논문 연계를 통해 무료로 제공되는 원문입니다.
서지반출

기타언어초록

Archaeal diversity during the composting process of pig manure and mushroom culture waste using a field-scale composter was investigated by a culture-independent approach. Metagenomes were isolated from six sampling sites at various stages and the 16S rRNA fragments were amplified with archaea-specific primers. Based on their rRNA sequences, a total of 180 clones, 30 clones from each site, could be divided into seven groups. Among them, group I to group V were assigned to the phylum Euryarchaeota, and group VI and group VII to the phylum Crenarchaeota. All the clones of group I (uncultured archaeon clone AE34) and group II (uncultured archaeon clone AS17) belonged to the class Methanomicrobia and the closest neighboring species was allocated to the Methanomicrobium mobile, a methanogen. Two major methanogen groups, I and II, were predominantly present at the initial stage and at the mid to late stages, respectively. The quantified values of their prevalence were 45.0% (81 out of a total of 180 clones) and 47.8% (86 of 180 clones), respectively. Group VI (an uncultured archaeon) and group VII (uncultured archaeon clone AF15) were minor groups and belonged to the class Thermoprotei. Little change in the methanogenic archaeal diversity was observed compared to that of the bacterial and fungal diversity. The results may help to gain information about $CH_4$ production from composting and to improve the fieldscale composter system, since the diversity would be dependent on the maturation and composition of the compost.